Le far-eastern blot est une technique de biologie moléculaire par transfert de molécules développée par T. Taki en 1994 pour analyser les lipides puis adaptée à d'autres molécules (métabolites, hormones, médicaments, etc.)↑ (en) Taki T., Handa S. and Ishikawa D., « Blotting of Glycolipids and Phospholipids from a High-Performance Thin-Layer Chromatogram to a Polyvinylidene Difluoride Membrane », Analytical Biochemistry, vol. 221, no 2,‎ septembre 1994, p.

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  • Le far-eastern blot est une technique de biologie moléculaire par transfert de molécules développée par T. Taki en 1994 pour analyser les lipides puis adaptée à d'autres molécules (métabolites, hormones, médicaments, etc.)
  • Far-Eastern blotting is a technique developed in 1994 by Taki and colleagues at the Tokyo Medical and Dental University, Japan for the analysis of lipids separated by high-performance thin layer chromatography (HPTLC). The lipids are transferred from the HPTLC plate to a PVDF membrane for further analysis, for example by enzymatic or ligand binding assays or mass spectrometry.Cholesterol, glycerophospholipids and sphingolipids are major constituents of the cell membrane and in certain cases function as second messengers in cell proliferation, apoptosis and cell adhesion in inflammation and tumor metastasis. Far-eastern blotting was established as a method for transferring lipids from an HPTLC plate to a polyvinyledene difluoride (PVDF) membrane within a minute. Applications of this with other methods have been studied. Far-eastern blotting allows for the following techniques: Purification of glycosphingolipids and phospholipids.Structural analysis of lipids in conjunction with direct mass spectrometry.Binding study using various ligands such as antibodies, lectins, bacterium, viruses, and toxins, andEnzyme reaction on membranes.Not only analysis of lipids but also metabolites of drugs and natural compounds from plants, and environmental hormones are possible by this method.
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  • Le far-eastern blot est une technique de biologie moléculaire par transfert de molécules développée par T. Taki en 1994 pour analyser les lipides puis adaptée à d'autres molécules (métabolites, hormones, médicaments, etc.)↑ (en) Taki T., Handa S. and Ishikawa D., « Blotting of Glycolipids and Phospholipids from a High-Performance Thin-Layer Chromatogram to a Polyvinylidene Difluoride Membrane », Analytical Biochemistry, vol. 221, no 2,‎ septembre 1994, p.
  • Far-Eastern blotting is a technique developed in 1994 by Taki and colleagues at the Tokyo Medical and Dental University, Japan for the analysis of lipids separated by high-performance thin layer chromatography (HPTLC).
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  • Far-eastern blot
  • Far-Eastern blotting
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